Sequencing and NGS

Our laboratory is equipped with technologies dedicated to the most efficient and innovative genomics. We are thus able to answer to all requests in this area, from robotic preparation of libraries for the high-throughput sequencing, including exomes sequencing projects, to targeted, genome and also metagenomic sequencing projects, de novo sequencing or mutation detection.

NovaSeq 6000, NextSeq 500, MiSeq, CBot – Illumina

Illumina offers innovative next-generation sequencing (NGS) platforms that deliver industry-leading data quality and accuracy, at an unparalleled scale.NGS involves rapid sequencing of large DNA stretches spanning entire genomes. The latest Illumina DNA sequencers enable a wide variety of uses, and can produce gigabases to over a terabase of data in a single sequencing run. By folowing the link here you will be able to consult the specificities of the Illumina sequencers.

Common DNA Sequencing Applications: Illumina sequencing enables a broad range of applications, such as whole-genome and de novo sequencing, targeted region sequencing, mutation discovery, and identification of copy number variations. Common DNA sequencing applications include:Whole Genome Sequencing: Characterize entire genomes of any size and complexity.

  • Exome sequencing: Sequence protein coding regions, as a cost-effective alternative to WGS.
  • Targeted sequencing: Sequence specific genes or other regions of interest.
  • De novo sequencing: Sequence and assemble novel genomes.
  • Mitochondrial sequencing: Detect mitochondrial disease-associated mutations.
  • Transcripome sequencing : transcriptome analysis
  • Epigenetics: methyl sequencing
  • Metagenomics: sequencing of 16S / 18S unti to characterize bacterial populations
  • Miseq : dedicated to small genome, amplicon and targeted gene panel sequencing

Nextseq500 and Hiseq 2500 and Hiseq 4000: dedicated to genome, exome, transcriptome sequencing


E220 – Covaris

The Covaris E220 is a targeted ultrasound device designed for the treatment of samples of many biological and chemical applications, including DNA and shear chromatin tissue homogenization, cell lysis, dissolution compound, and micronization particles.

The use of acoustic energy (Adaptive Focused Acoustics ™) allows processing of 1-96 samples with precision and accuracy to samples of variable volumes.

STARlet – Hamilton, Bravo – Agilent & Sciclone - Perkin Elmer

The STARlet (Hamilton) workstation, the Bravo Automated Liquid Handling Platform (Agilent) and the Sciclone® G3 NGSx iQ™ workstation (Perkin Elmer) are liquid Handling workstations providing reproductibility and flexibility. Both dedicated to NGS libraries preparation, these equipments perform a large panel of automation protocols for Illumina Sequencing as libraries TruSeq DNA and RNA, Kapa DNA, Haloplex (Agilent), and WES or targeted captures (Nimblegen Roche, Sureselect Agilent) …

Chemagic Prime - Perkin Elmer

The chemagic Prime™ instrument is a highly automated platform combining chemagen patented M-PVA Magnetic Bead technology for DNA and RNA purification with liquid handling to provide high-throughput automated isolation of ultra-pure nucleic acids. The liquid handling unit transfers the primary samples to plates suitable for automated nucleic acid purification which are in turn automatically loaded on the extraction deck.

LabChip GX Touch Nucleic Acid Analyzer - Perkin Elmer

DNA and RNA quantitation and sizing can be done in seconds using automated capillary electrophoresis separation. The LabChip® GX Touch™ nucleic acid analyzer’s microfluidic technology generates reproducible, high-resolution data and is optimal for :
• NGS library preparation (smear and fragment analysis) and quality control
• RNA and DNA fragment analysis (including cell-free (cfDNA), FFPE, liquid biopsy, and PCR-free samples)
• Quantitation and qualification for CRISPR fragment analysis
• Agarose gel workflows

Bioanalyzer 2100 – Agilent

The Agilent 2100 bioanalyzer is the industry standard for RNA sample QC and has replaced labor-intensive gel electrophoresis for this application. It is also rapidly replacing gel electrophoresis for DNA fragment analysis and SDS-PAGE analysis of protein samples.