The PamStation 12 is a fully automated instrument designed for processing PamChip 4 microarrays. It is available with either phosphotyrosine kinase (PTK) or serine-threonin kinase (STK) arrays. The 3D surface of the PamChip microarrays provides unique characteristics to allow real-time analysis of nucleic acid and protein binding for allows enzymatic reactions of kinases to be followed kinetically.
The sample consisting of protein and labelled antibodies are dispensed onto the PamChip, which is placed in the PamStation12. Once inside the instrument the sample is hybridized or incubated at a pre-determined temperature. During hybridization or incubation the sample is pumped back and forth through the porous material to maximize binding kinetics and minimize assay time. Reaction times are typically minutes to an hour.